Facilities

Orbitrap Elite -TFS- 2012

Installation date: February 2012

Application

  • The Orbitrap Elite is a hybrid system composed with a linear ion trap (LTQ Velos Pro) and an Orbitrap cell. Thanks to its scan speed and sensitivity, the instrument allow the analysis of complex mixtures of proteins.
  • Connected with a nano-chromatography system using C18 column (75 µm x 50 cm) , from 2000 to 4000 proteins are identified in 2 hours runs.
  • The sequence information are obtained after CID, HCD or ETD fragmentation.
  • The instrument was acquired thanks to the financial support of the Fondation ARC pour la Recherche sur le Cancer and INSERM.

Orbitrap Specifications

  • Resolution: 120 000 (M/ΔM) with standard MS scan, up to 240 000 (M/ΔM)  à 400 m/z
  • Accuracy: <10 ppm with external calibration
  • Sensitivity with nanoLC in the range of fentomole
  • Dynamic range on 3 to 5 magnitudes
  • scan speed between 0.2 and 2 seconds depending on the resolution

Linear trap specifications

  • Resolution: 1000 (M/ΔM) in rapid scan mode
  • Accuracy: between 0.15 and 0.6 Da in rapid scan mode
  • Scan speed: around 0.1 second in rapid scan mode

Fragmentation modes

  • CID
  • ETD
  • HCD

orbitrap elite.jpg

QTOF Xevo  –- Waters - 2015

Installation date: March 2015

Application

  • The QTOF Xevo is equipped with a nanospray source and a standard ESI source. The chromatographic system (Acquity class M) is used for both nanoLC (C18 column, 75µm x 25 cm) and more conventional column (1 mm I.D.). The QTOF Xevo is used for the analysis of protein with LC-MS (C4 columns) and the quantification of peptides and little molecules

Specifications

  • Resolution: 30000 (M/ΔM) in standard MS scan
  • -Accuracy: <10 ppm with external calibration
  • NanoLC MS sensitivity in the range of femtomole (peptide)
  • Scan speed from 0.1 to 1 second depending on sensitivity

QTOF Xevo.jpg

Last update on 03/28/2019

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